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An authoritative technical evaluation for procurement officers, pharmaceutical researchers, diagnostic manufacturers, and commercial chemical buyers worldwide.
Reverse Triiodothyronine (rT3), systematically designated as 3,3',5'-Triiodo-L-thyronine (CAS Registry Number: 5817-39-0), is an isomer of the primary active thyroid hormone 3,5,3'-triiodothyronine (T3). In cellular metabolic control, rT3 serves as the primary biologically inactive metabolite resulting from the inner-ring deiodination of thyroxine (T4) by the enzyme Type 3 iodothyronine deiodinase (DIO3). Understanding the chemical purity, synthesis route, structural integrity, and supply chain logistics of Reverse T3 API is paramount for diagnostic assay manufacturers, clinical assay standard formulation, and advanced endocrine research pipelines.
Unlike active T3 which binds to nuclear thyroid hormone receptors (TRα and TRβ) with high affinity to stimulate oxygen consumption and metabolic rate, Reverse T3 acts as a competitive antagonist at cellular receptor interfaces and membrane transporters (such as MCT8 and OATP1C1). Buying rT3 requiring high HPLC analytical verification (≥98.0% purity) is crucial because minor isomeric contamination by active T3 (3,5,3'-triiodothyronine) can lead to false positives in competitive immunoassay calibration, cell line metabolic assays, and quantitative mass spectrometry (LC-MS/MS) methodologies.
Below is the validated analytical specification for high-purity Reverse Triiodothyronine active raw material supplied by certified global exporters:
| Chemical Metric / Parameter | Specification & Standard Value | Analytical Method / Protocol |
|---|---|---|
| Chemical Name | O-(4-Hydroxy-3,5-diiodophenyl)-3-iodo-L-tyrosine / Reverse T3 | IUPAC Nomenclature |
| CAS Registry Number | 5817-39-0 | Chemical Abstracts Service |
| Molecular Formula | C15H12I3NO4 | High-Resolution Mass Spec (HRMS) |
| Molecular Weight | 650.97 g/mol | Mass Spectrometry Validation |
| Assay / Purity Grade | ≥ 98.5% (HPLC Peak Area Normalized) | Reverse-Phase High Performance Liquid Chromatography |
| Appearance | Off-white to light beige crystalline powder | Visual Inspection |
| Specific Optical Rotation | [α]D20 = +18.0° to +22.0° (c=1 in ethanol/1M HCl) | Polarimetry |
| Residual Solvents | Complies with ICH Q3C Guidelines (Methanol < 3000ppm, Ethanol < 5000ppm) | Gas Chromatography with Headspace (GC-HS) |
| Loss on Drying (LOD) | ≤ 1.0% | Thermogravimetric Analysis / Vacuum Drying |
| Recommended Storage | -20°C, Desiccated, Protected from Light under Inert Nitrogen Blanket | Stability Testing Protocol (ICH Q1A) |
Thyroxine ($T_4$) contains four iodine atoms: two on the tyrosyl (outer) ring and two on the phenolic (inner) ring. The peripheral metabolism of $T_4$ undergoes two mutually exclusive pathways depending on regional enzymatic activation:
When biological systems experience severe physiological stress, starvation, critical illness, systemic inflammation, or trauma—often referred to as Non-Thyroidal Illness Syndrome (NTIS) or Euthyroid Sick Syndrome—DIO1 activity decreases while DIO3 activity increases. This shifts $T_4$ conversion away from metabolic $T_3$ toward Reverse $T_3$, preserving cellular energy stores. Consequently, supplying high-purity rT3 reference standards to diagnostic laboratories enables precise monitoring of endocrine stress markers and metabolic dysfunction.
Overcoming global sourcing bottlenecks, regulatory barriers, and cold-chain distribution challenges for enterprise-level pharmaceutical procurement.
Our collaborative industrial infrastructure spans over 250,000 square meters, providing scalable synthesis from gram-scale specialized research batches to multi-kilogram commercial production of APIs, thyroid hormones, and customized peptides under strict cGMP environments.
We provide global partners with complete analytical documentation packages including Certificate of Analysis (CoA), HPLC chromatograms, Mass Spectrometry (MS) spectra, Proton/Carbon NMR analysis, stability data, and Drug Master File (DMF) filing support for FDA, EMA, and NMPA filings.
Reverse T3 and delicate amino acid derivatives require strict temperature management to prevent oxidative deiodination. Our global logistics team manages dry-ice cold-chain shipping with real-time temperature tracking to ensure 100% material integrity upon receipt.
Meeting regional pharmacopeial standards across North America, Europe, Latin America, and Africa with customized localized support.
The global demand for thyroid hormone APIs and specialized metabolites like Reverse T3 has expanded rapidly, driven by the growth of chemiluminescence immunoassay (CLIA) diagnostic kits, Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) reference laboratory testing, and biopharmaceutical formulation development. Gentolex has established localized support channels to streamline regulatory clearance, customs documentation, and commercial delivery across key international territories.
Clients in the United States, Canada, and EU member states demand strict compliance with USP, EP, and ICH guidelines. Our Reverse T3 raw materials undergo rigorous testing for heavy metal residues, elemental impurities (ICH Q3D), and microbial limits. We support institutional procurement with formal quality agreements, REACH compliance for Europe, and seamless customs classification under HS Code 2937.50.
Recognizing the expanding biopharmaceutical infrastructure in emerging markets, Gentolex has established dedicated direct representative hubs in Mexico and South Africa. These local presences provide bilingual technical support, localized customs facilitation, fast-track delivery options, and simplified currency invoicing for regional diagnostic manufacturers and research universities.
How high-grade Reverse Triiodothyronine is utilized in commercial diagnostic, industrial, and clinical scientific environments.
Clinical diagnostic laboratories rely on isotopic and high-purity non-labeled Reverse T3 standard solutions to establish linear calibration curves for LC-MS/MS equipment. High analytical purity ensures precise separation of rT3 from isobaric T3 fragments without cross-talk interference.
Manufacturers of Radioimmunoassay (RIA), Enzyme-Linked Immunosorbent Assay (ELISA), and Chemiluminescent Immunoassay (CLIA) diagnostic kits purchase bulk rT3 for hapten-conjugate synthesis, antibody validation, and quality control serum matrix spiking.
Academic institutions and CROs utilize rT3 to study deiodinase enzyme kinetics (DIO1, DIO2, DIO3), transmembrane transport mechanisms via Monocarboxylate Transporter 8 (MCT8), and hepatic metabolic signaling under hypoxic or caloric-restricted conditions.
Next-generation enzymatic iodination, automated continuous flow reactors, and AI-driven quality assurance.
The manufacturing landscape for halogenated aromatic amino acids and thyroid derivatives is undergoing a paradigm shift. Traditional multi-step chemical synthesis pathways—which often rely on harsh halogenating agents and generate toxic chemical waste—are being upgraded with sustainable chemical engineering protocols. Our technology roadmap outlines the technological advancements powering our modern peptide and chemical synthesis facilities:
By leveraging engineered haloperoxidase enzymes, our future-ready synthetic pipelines achieve 100% regioselectivity when adding iodine atoms to the phenolic ring. This eliminates structural isomers, drastically reduces purification steps, and guarantees batch-to-batch consistency for Reverse T3 synthesis.
Integrating machine learning algorithms directly into online preparative HPLC systems allows real-time fraction collection optimization. AI sensors detect micro-elution shifts in real time, preventing trace impurity tailing and delivering API purities exceeding 99.5% with zero human error.
Gentolex’s overarching mission is to create unprecedented opportunities by connecting the global healthcare and scientific community with superior services and guaranteed chemical products. Up to date, Gentolex Group has proudly served esteemed clients from more than 10 countries worldwide, establishing strong commercial hubs and direct local representative offices in Mexico and South Africa.
Our core capabilities focus on supplying high-purity peptide APIs and Custom Peptides synthesis, Finished Dosage Form (FDF) licensing out, technical support & consultation, product line installation, laboratory setup, and end-to-end global sourcing & supply chain management solutions.
Our main services focus on supplying peptides APIs and Custom Peptides, FDF license out, Technical Support & Consultation, Product Line and Lab Setup, Sourcing & Supply Chain Solutions.
An overall factory construction area of 250,000 square meters under international standard to offer flexible, scalable and cost-effective solutions.
Gentolex offers an extensive range of APIs and intermediates for development study and commercial application with cGMP standard from long-term collaborations. Documents and Certificates are supported to customers worldwide.
State-of-the-art synthetic process optimization, custom formulation design, analytical method validation, and technology transfer for pharmaceutical innovators.
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Complete product offerings available for global commercial export with certified documentation.
Addressing key technical, regulatory, purchasing, and logistical inquiries regarding Reverse T3 (rT3) suppliers and exporters.
Gentolex integrates state-of-the-art cGMP manufacturing facilities spanning over 250,000 square meters with rigorous double-testing protocols (HPLC, HRMS, NMR). We offer validated batch-to-batch purity (≥98.5%), complete DMF documentation support, fast cold-chain export, and local customer service centers in key international hubs including Mexico and South Africa.
The CAS Registry Number for Reverse Triiodothyronine (rT3) is 5817-39-0. Its molecular formula is C15H12I3NO4, with a molecular weight of 650.97 g/mol. Its IUPAC name is (2S)-2-amino-3-[4-(4-hydroxy-3,5-diiodophenoxy)-3-iodophenyl]propanoic acid.
Active T3 (3,5,3'-triiodothyronine) possesses two iodine atoms on its inner tyrosyl ring and one on its outer phenolic ring. Reverse T3 (3,3',5'-triiodothyronine) has one iodine on the inner ring and two on the outer ring. Biologically, active T3 binds strongly to nuclear receptors to increase metabolic rate, whereas Reverse T3 is metabolic-inactive and acts as a competitive feedback regulator.
We accommodate flexible ordering scales ranging from analytical standard quantities (100mg to 1g research vials) up to bulk commercial manufacturing batches (100g, 1kg, and multi-kilogram drums). Customized packaging in light-resistant amber glass bottles under argon flushing is standard for all orders.
Every shipment is accompanied by a batch-specific Certificate of Analysis (CoA) including reverse-phase HPLC chromatograms verifying purity, Mass Spectrometry (MS) structural validation, Proton NMR spectrum, Karl Fischer titration for water content, optical rotation data, and residual solvent GC reports conforming to ICH guidelines.
Reverse T3 powder should be stored at -20°C in a desiccated, dark environment protected from moisture and light exposure. When handled in solution, it is advisable to use nitrogen-blanketed solvent systems to prevent deiodination or oxidative degradation over time.
We utilize validated temperature-controlled packaging with dry ice or phase-change thermal packs via express air carriers (DHL, FedEx, specialized chemical logistics). Temperature dataloggers are included upon request to verify continuous thermal protection throughout international transit.
Yes. Gentolex has established dedicated direct representatives and operational hubs in Mexico and South Africa. Our local teams assist with import permits, sanitary clearance certificates (COFEPRIS/SAHPRA), localized customs clearance, and DDP (Delivered Duty Paid) shipping options.
Yes. In addition to standard unlabeled Reverse T3 API, our custom peptide and chemical synthesis division offers stable isotope-labeled analogues (such as 13C-labeled or Deuterium-labeled rT3) designed specifically for quantitative LC-MS/MS internal standard calibration assays.
You can submit your technical specifications, required purity, quantity, and delivery destination directly to our international sales team through our product contact portals. Quotations, technical data sheets, and sample availability are typically provided within 24 business hours.